目的 研究土茯苓及其混淆品之间的化学成分差异,为土茯苓类商品药材的品种鉴别和质量评价提供依据。方法 采用HPLC指纹图谱方法,对正品土茯苓【光叶菝葜(Smilax glabra)根茎】、华肖菝葜(Heterosmilax chinensis)和短柱肖菝葜(H. yunnanensis)的根茎采集品,以及购自全国各地共102份土茯苓类药材进行研究,利用相似度评价及主成分分析对图谱数据进行分析。结果 建立了土茯苓HPLC指纹图谱的分析方法,通过对光叶菝葜、华肖菝葜和短柱肖菝葜的检测,分别标定11个、15个和8个共有峰,指纹图谱显示3个品种的共有峰仅有5个,分别为1、2、3、13、14号色谱峰。共指认了3种指纹图谱中的12个色谱峰,其中光叶菝葜中指认了9个色谱峰,4种成分为首次指认,并首次指认了华肖菝葜和短柱肖菝葜中的6种和2种成分。对峰面积进行主成分分析结果表明,3个品种间的化学成分存在较明显差异,可将3个不同品种区分开。将建立的HPLC方法应用于商品药材的分析,鉴定收集到的102份商品药材中有光叶菝葜65份、短柱肖菝葜17份、华肖菝葜20份。结论 该指纹图谱分析方法简便、可靠,指纹图谱的构建和化学模式识别可为土茯苓药材与混淆品的鉴别提供方法和依据。
Abstract
OBJECTIVE To investigate the chemical differences between Smilacis Glabrae Rhizoma and its adulterants, and provide a basis for the identification and quality evaluation of Tufuling samples purchased from the markets.METHODS The method of HPLC fingerprinting was used to analyze Smilacis Glabrae Rhizoma, Heterosmilacis Chinensis Rhizoma, Heterosmilacis Yunnanensis Rhizoma, and 102 Tufuling samples which were collected around China. The fingerprints were analyzed by the methods of similarity and principal component analysis (PCA).RESULTS The analytical method of HPLC fingerprinting was established. Eleven, fifteen and eight common peaks were selected in the fingerprints of Smilacis Glabrae Rhizoma, Heterosmilacis Chinensis Rhizoma, and Heterosmilacis Yunnanensis Rhizoma, respectively. Only five common peaks were found in the fingerprints of the three species, which were No. 1, 2, 3, 13, and 14 peaks. A total of twelve peaks were characterized in the three fingerprints. Nine peaks were characterized in the fingerprint of Smilacis Glabrae Rhizoma, among which, four constituents were characterized for the first time. Six and two constituents were for the first time characterized in the fingerprints of Heterosmilacis Chinensis Rhizoma and Heterosmilacis Yunnanensis Rhizoma, respectively. The result of PCA analysis showed that the chemical differences between the three species were quite obvious and they could be distinguished from each other. The established method was used for the analysis of Tufuling samples purchased from the markets. Sixty-five samples were identified as Smilacis Glabrae Rhizoma, seventeen samples were identified as Heterosmilacis Yunnanensis Rhizoma, and twenty samples were identified as Heterosmilacis Chinensis Rhizoma.CONCLUSION The established method is simple and reliable, and the development of fingerprint and its chemical pattern recognition provide the way and basis for identification of Smilacis Glabrae Rhizoma and its adulterants.
关键词
光叶菝葜 /
HPLC指纹图谱 /
华肖菝葜 /
短柱肖菝葜 /
商品鉴别 /
土茯苓
{{custom_keyword}} /
Key words
Smilax glabra /
HPLC fingerprint /
Heterosmilax chinensis /
Heterosmilax yunnanensis /
commercial samples identification /
Smilacis Glabrae Rhizoma
{{custom_keyword}} /
中图分类号：
R284
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]Ch. P(2015) Vol Ⅰ(中国药典2015年版. 一部)[S]. 2015:18.[2]SONG L, WU Y, HU L, et al. Zhong Hua Ben Cao(中华本草)[M]. Vol 22. Shanghai:Shanghai Science and Technology Publisher, 1999:161-164.[3]XIAO P G. Modern Chinese Material Medica(新编中药志)[M]. Vol 1. Beijing:Chemical Industry Press, 2001:60-65.[4]Nanjing University of Chinese Medicine. The Dictionary of Chinese Herbal Medicine(中药大辞典)[M]. Shanghai:Shanghai Science and Technology Publisher, 2006:122-125.[5]DU Q Z, LI L, JERZ G. Purification of astilbin and isoastilbin in the extract of Smilax glabra rhizome by high-speed counter-current chromatography[J]. J Chromatogr A, 2005, 1077(1):98-101.[6]XIAO J, WEI F, LIN R C. Advance on the study of the genns smilax[J]. Chin Pharm J(中国药学杂志),2001,36(6):369-372.[7]CHEN T, LI J X, XU Q. Phenylpropanoid glycosides from Smilax glabra[J]. Phytochemistry, 2000, 53(8):1051-1055.[8]YI Y J, CAO Z Z, YANG W H, et al. Chemical studies of Smilax glabra Ⅲ). Isolation and identification of smiglanin from Smilax glabra[J]. Acta Pharm Sin(药学学报), 1995, 30(9):718-720.[9]ZHANG B J, LIU O Y, LIU L, et al. Study on the anti-inflammatory, analgesic and diuretic effects of Rhizoma Smilacis Glabrae and astilbin[J]. Pharmacol Clin Chin Mater Clin Med(中药药理与临床), 2004, 20(1):11-12.[10]KUSPRADINI H, MITSUNAGA T, OHASHI H. Antimicrobial activity against Streptococcus sobrinus and glucosyltransferase inhibitory activity of taxifolin and some flavanonol rhamnosides from kempas (Koompassia malaccensis) extracts[J]. J Wood Sci, 2009, 55(4):308-313.[11]CAI Y, CHEN T, XU Q. Astilbin suppresses collagen-induced arthritis via the dysfunction of lymphocytes[J]. Inflamm Res, 2003, 52(8):334-340.[12]ZHANG Q F, ZHANG Z R, CHEUNG H Y. Antioxidant activity of Rhizoma Smilacis Glabrae extracts and its key constituent-astilbin[J]. Food Chem, 2009, 115(1):297-303.[13]YI H W, LU X M, FANG F. Astilbin inhibits the adhesion of T lymphocytes via decreasing TNF-α and its associated MMP-9 activity and CD44 expression[J]. Int Immunopharmacol, 2008, 8(10):1467-1474.[14]XU G J, XU L S. Research on the Varieties and Quality of Traditional Chinese Medicine Southern Cooperative Group(常用中药材品种整理和质量研究 南方协作组 第二册)[M]. Vol 2. Fuzhou:Fujian Science and Technology Publisher, 1997:504-520.[15]Standard of Traditional Chinese Medicine in Sichuan Province (1987)(四川省中药材标准 1987年版)[S]. 1987:69-70.[16]Standard of Traditional Chinese Medicine Quality in Guizhou Province (1988)(贵州省中药材质量标准 1988年版)[S]. 1988:46.[17]Standard of Traditional Chinese Medicine Quality in Hunan Province (1993)(湖南省中药材标准 1993年版)[S]. 1993:9-10.[18]XIE P S, CHEN S B, LIANG Y Z, et al. Chromatographic fingerprint analysis-a rational approach for quality assessment of traditional Chinese herbal medicine[J]. J Chromatogr A, 2006, 1112(1-2):171-180.[19]XU S, SHANG M Y, LIU G X, et al. Chemical constituents from the rhizomes of Smilax glabra and their antimicrobial activity[J]. Molecules, 2013, 18(5):5265-5287.[20]XU S, SHANG M Y, LIU G X, et al. Simultaneous determination of seven bioactive constituents in Smilacis Glabrae Rhizoma by high-performance liquid chromatography[J].China J Chin Mater Med(中国中药杂志), 2015, 40(3):469-479.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
“重大新药创制”科技重大专项资助项目(2013ZX09508104);北京市自然科学基金资助项目(7162111)
{{custom_fund}}
PDF(1872 KB)
